14 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Midway | 1 | 21,148 | 19,311 | 1.61% | 1,760 | 12 | CDP |
| Quincy | 2 | 8,174 | 8,032 | 0.68% | 702 | 12 | City |
| Quincy | 3 | 8,174 | 7,896 | 0.68% | 702 | 12 | City |
| Midway | 4 | 3,472 | 3,548 | -0.37% | 361 | 10 | City |
| Midway | 5 | 3,472 | 3,537 | -0.37% | 361 | 10 | City |
| Chattahoochee | 6 | 3,053 | 2,960 | 0.83% | 545 | 6 | City |
| Chattahoochee | 7 | 3,053 | 2,927 | 0.83% | 545 | 6 | City |
| Havana | 8 | 1,758 | 1,754 | 0.11% | 631 | 3 | Town |
| Havana | 9 | 1,758 | 1,748 | 0.11% | 631 | 3 | Town |
| Midway | 10 | 1,363 | 1,417 | -1.59% | 1,849 | 1 | CDP |
| Gretna | 11 | 1,284 | 1,287 | 0% | 150 | 9 | City |
| Gretna | 12 | 1,284 | 1,283 | 0% | 150 | 9 | City |
| Greensboro | 13 | 465 | 460 | 0.22% | 202 | 2 | Town |
| Greensboro | 14 | 465 | 458 | 0.22% | 202 | 2 | Town |
Midway is the largest city in Gadsden County, with a population of 21,148.
Greensboro is the smallest city in Gadsden County, with a population of 465.
There are 14 cities in Gadsden County, Florida.