10 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Bonifay | 1 | 2,856 | 2,768 | 0.63% | 607 | 5 | City |
| Bonifay | 2 | 2,856 | 2,765 | 0.63% | 607 | 5 | City |
| Ponce Leon | 3 | 522 | 502 | 0.77% | 105 | 5 | Town |
| Ponce Leon | 4 | 522 | 501 | 0.77% | 105 | 5 | Town |
| Esto | 5 | 352 | 340 | 0.57% | 162 | 2 | Town |
| Esto | 6 | 352 | 339 | 0.57% | 162 | 2 | Town |
| Westville | 7 | 270 | 260 | 0.75% | 37 | 7 | Town |
| Westville | 8 | 270 | 260 | 0.75% | 37 | 7 | Town |
| Noma | 9 | 216 | 208 | 0.47% | 202 | 1 | Town |
| Noma | 10 | 216 | 208 | 0.47% | 202 | 1 | Town |
Bonifay is the largest city in Holmes County, with a population of 2,856.
Noma is the smallest city in Holmes County, with a population of 216.
There are 10 cities in Holmes County, Florida.