11 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Marianna | 1 | 8,397 | 6,286 | 5.29% | 451 | 19 | City |
| Graceville | 2 | 2,236 | 2,149 | 0.77% | 517 | 4 | City |
| Sneads | 3 | 1,799 | 1,702 | 1.07% | 409 | 4 | Town |
| Malone | 4 | 1,596 | 1,826 | -2.8% | 524 | 3 | Town |
| Grand Ridge | 5 | 934 | 886 | 0.97% | 215 | 4 | Town |
| Cottondale | 6 | 886 | 849 | 0.8% | 271 | 3 | Town |
| Greenwood | 7 | 565 | 543 | 0.71% | 124 | 5 | Town |
| Alford | 8 | 525 | 492 | 1.16% | 498 | 1 | Town |
| Jacob City | 9 | 233 | 217 | 1.3% | 76 | 3 | City |
| Campbellton | 10 | 205 | 193 | 0.99% | 79 | 3 | Town |
| Bascom | 11 | 94 | 88 | 1.08% | 392 | 0 | Town |
Marianna is the largest city in Jackson County, with a population of 8,397.
Bascom is the smallest city in Jackson County, with a population of 94.
There are 11 cities in Jackson County, Florida.