31 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Clermont | 1 | 52,728 | 43,340 | 3.69% | 2,926 | 18 | City |
| Leesburg | 2 | 40,487 | 27,127 | 7.07% | 1,029 | 39 | City |
| Groveland | 3 | 26,030 | 18,887 | 5.8% | 1,229 | 21 | City |
| Eustis | 4 | 25,525 | 23,233 | 1.83% | 2,211 | 12 | City |
| Tavares | 5 | 22,754 | 19,019 | 3.39% | 1,844 | 12 | City |
| Minneola | 6 | 21,043 | 14,201 | 6.95% | 1,890 | 11 | City |
| Mount Dora | 7 | 18,725 | 16,405 | 2.54% | 2,129 | 9 | City |
| Lady Lake | 8 | 17,603 | 16,028 | 1.82% | 1,885 | 9 | Town |
| Mascotte | 9 | 9,753 | 6,737 | 6.59% | 757 | 13 | City |
| Fruitland Park | 10 | 9,095 | 8,430 | 1.48% | 1,280 | 7 | City |
| Mount Plymouth | 11 | 4,995 | 4,812 | 1.34% | 1,736 | 3 | CDP |
| Umatilla | 12 | 4,033 | 3,747 | 1.43% | 1,105 | 4 | City |
| West DeLand | 13 | 3,584 | 3,271 | 0.11% | 1,667 | 2 | CDP |
| Lake Mack-Forest Hills | 14 | 2,880 | 2,425 | 4.54% | 595 | 5 | CDP |
| Silver Lake | 15 | 2,685 | 2,225 | 2.01% | 1,548 | 2 | CDP |
| Lake Kerr | 16 | 2,473 | 1,851 | 7.15% | 250 | 10 | CDP |
| Astatula | 17 | 2,357 | 1,899 | 4.02% | 752 | 3 | Town |
| Montverde | 18 | 1,848 | 1,663 | 2.04% | 998 | 2 | Town |
| Howey-in-the-Hills | 19 | 1,705 | 1,643 | 0.71% | 542 | 3 | Town |
| Astor | 20 | 1,447 | 1,242 | -0.48% | 619 | 2 | CDP |
| Yalaha | 21 | 1,414 | 1,547 | 0.21% | 249 | 6 | CDP |
| Pine Lakes | 22 | 1,377 | 1,127 | 2.53% | 860 | 2 | CDP |
| DeLand Southwest | 23 | 1,278 | 1,372 | 1.19% | 2,092 | 1 | CDP |
| North DeLand | 24 | 1,162 | 1,119 | -1.69% | 2,213 | 1 | CDP |
| Paisley | 25 | 1,134 | 1,301 | 1.89% | 362 | 3 | CDP |
| Lake Kathryn | 26 | 995 | 737 | 0.51% | 355 | 3 | CDP |
| Ferndale | 27 | 647 | 518 | 1.89% | 241 | 3 | CDP |
| Lisbon | 28 | 246 | 479 | -0.4% | 137 | 2 | CDP |
| Pittman | 29 | 223 | 249 | 1.36% | 177 | 1 | CDP |
| Okahumpka | 30 | 208 | 143 | -1.89% | 842 | 0 | CDP |
| Altoona | 31 | 32 | 77 | 6.67% | 71 | 0 | CDP |
Clermont is the largest city in Lake County, with a population of 52,728.
Altoona is the smallest city in Lake County, with a population of 32.
There are 31 cities in Lake County, Florida.