42 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Cape Coral | 1 | 242,422 | 194,001 | 4.03% | 2,284 | 106 | City |
| Cape Coral | 2 | 242,422 | 195,436 | 4.03% | 2,284 | 106 | City |
| Lehigh Acres | 3 | 133,854 | 124,523 | 2.4% | 1,444 | 93 | CDP |
| Fort Myers | 4 | 103,075 | 86,428 | 3.16% | 2,577 | 40 | City |
| Fort Myers | 5 | 103,075 | 87,288 | 3.16% | 2,577 | 40 | City |
| Bonita Springs | 6 | 57,918 | 53,665 | 1.41% | 1,508 | 38 | City |
| Bonita Springs | 7 | 57,918 | 53,887 | 1.41% | 1,508 | 38 | City |
| North Fort Myers | 8 | 43,263 | 42,315 | 0.6% | 876 | 49 | CDP |
| Estero | 9 | 38,790 | 36,948 | 0.9% | 1,592 | 24 | Village |
| Estero | 10 | 38,790 | 37,055 | 0.9% | 1,592 | 24 | Village |
| San Carlos Park | 11 | 19,404 | 18,256 | 0.89% | 4,108 | 5 | CDP |
| Villas | 12 | 14,359 | 14,421 | 1.31% | 3,105 | 5 | CDP |
| Iona | 13 | 14,026 | 13,588 | -0.64% | 2,117 | 7 | CDP |
| Cypress Lake | 14 | 12,430 | 12,766 | 0.31% | 3,245 | 4 | CDP |
| Gateway | 15 | 10,893 | 10,552 | 1.55% | 2,030 | 5 | CDP |
| McGregor | 16 | 7,711 | 7,899 | 0.26% | 3,259 | 2 | CDP |
| Three Oaks | 17 | 6,797 | 5,362 | 3.25% | 3,920 | 2 | CDP |
| Sanibel | 18 | 6,350 | 6,385 | -0.13% | 392 | 16 | City |
| Sanibel | 19 | 6,350 | 6,389 | -0.13% | 392 | 16 | City |
| Fort Myers Shores | 20 | 6,116 | 6,745 | 0.69% | 2,949 | 2 | CDP |
| Tice | 21 | 5,718 | 5,379 | 1.49% | 4,789 | 1 | CDP |
| Lochmoor Waterway Estates | 22 | 5,608 | 4,995 | 1.7% | 2,341 | 2 | CDP |
| Whiskey Creek | 23 | 5,464 | 5,508 | 1% | 3,537 | 2 | CDP |
| Fort Myers Beach | 24 | 5,239 | 5,581 | -1.3% | 1,883 | 3 | Town |
| Fort Myers Beach | 25 | 5,239 | 5,583 | -1.3% | 1,883 | 3 | Town |
| Florida Gulf Coast University | 26 | 4,741 | 2,507 | 7.14% | 1,966 | 2 | CDP |
| Buckingham | 27 | 4,351 | 4,269 | 0.48% | 242 | 18 | CDP |
| Pine Manor | 28 | 4,073 | 4,582 | 1.07% | 8,816 | 0 | CDP |
| St. James City | 29 | 3,422 | 3,510 | -0.7% | 234 | 15 | CDP |
| Alva | 30 | 3,084 | 3,013 | 1.08% | 177 | 17 | CDP |
| Olga | 31 | 2,953 | 2,773 | 2.32% | 701 | 4 | CDP |
| Bokeelia | 32 | 2,342 | 1,536 | 1.61% | 262 | 9 | CDP |
| Verandah | 33 | 2,144 | 1,291 | 7.15% | 1,007 | 2 | CDP |
| Harlem Heights | 34 | 1,624 | 1,362 | -1.4% | 2,510 | 1 | CDP |
| Pine Island Center | 35 | 1,569 | 2,255 | -1.2% | 359 | 4 | CDP |
| Punta Rassa | 36 | 1,275 | 1,566 | -2.45% | 534 | 2 | CDP |
| Palmona Park | 37 | 1,104 | 754 | -0.18% | 1,341 | 1 | CDP |
| Page Park | 38 | 942 | 621 | 3.18% | 3,502 | 0 | CDP |
| Matlacha | 39 | 877 | 853 | 1.5% | 4,407 | 0 | CDP |
| Pineland | 40 | 372 | 266 | -0.53% | 415 | 1 | CDP |
| Matlacha Isles-Matlacha Shores | 41 | 63 | 339 | -14.86% | 315 | 0 | CDP |
| Captiva | 42 | 43 | 165 | -45.57% | 36 | 1 | CDP |
Cape Coral is the largest city in Lee County, with a population of 242,422.
Captiva is the smallest city in Lee County, with a population of 43.
There are 42 cities in Lee County, Florida.