6 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Madison | 1 | 3,014 | 2,910 | 0.67% | 1,143 | 3 | City |
| Madison | 2 | 3,014 | 2,912 | 0.67% | 1,143 | 3 | City |
| Greenville | 3 | 780 | 747 | 0.78% | 592 | 1 | Town |
| Greenville | 4 | 780 | 747 | 0.78% | 592 | 1 | Town |
| Lee | 5 | 383 | 366 | 0.79% | 157 | 2 | Town |
| Lee | 6 | 383 | 366 | 0.79% | 157 | 2 | Town |
Madison is the largest city in Madison County, with a population of 3,014.
Lee is the smallest city in Madison County, with a population of 383.
There are 6 cities in Madison County, Florida.