8 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Yulee | 1 | 14,347 | 13,346 | 1.34% | 619 | 23 | CDP |
| Fernandina Beach | 2 | 13,817 | 13,044 | 1.06% | 1,167 | 12 | City |
| Fernandina Beach | 3 | 13,817 | 13,090 | 1.06% | 1,167 | 12 | City |
| Nassau Village-Ratliff | 4 | 4,328 | 4,789 | -1.55% | 295 | 15 | CDP |
| Hilliard | 5 | 3,105 | 2,961 | 0.91% | 564 | 6 | Town |
| Hilliard | 6 | 3,105 | 2,963 | 0.91% | 564 | 6 | Town |
| Callahan | 7 | 1,847 | 1,524 | 3.47% | 1,022 | 2 | Town |
| Callahan | 8 | 1,847 | 1,537 | 3.47% | 1,022 | 2 | Town |
Yulee is the largest city in Nassau County, with a population of 14,347.
Callahan is the smallest city in Nassau County, with a population of 1,847.
There are 8 cities in Nassau County, Florida.