37 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Deltona | 1 | 102,129 | 93,700 | 1.61% | 2,736 | 37 | City |
| Deltona | 2 | 102,129 | 94,046 | 1.61% | 2,736 | 37 | City |
| Daytona Beach | 3 | 89,444 | 71,599 | 3.99% | 1,327 | 67 | City |
| Daytona Beach | 4 | 89,444 | 72,298 | 3.99% | 1,327 | 67 | City |
| Port Orange | 5 | 67,511 | 62,621 | 1.43% | 2,516 | 27 | City |
| Port Orange | 6 | 67,511 | 62,734 | 1.43% | 2,516 | 27 | City |
| DeLand | 7 | 47,427 | 37,791 | 4.04% | 2,438 | 19 | City |
| DeLand | 8 | 47,427 | 38,225 | 4.04% | 2,438 | 19 | City |
| Ormond Beach | 9 | 44,964 | 43,073 | 0.83% | 1,286 | 35 | City |
| Ormond Beach | 10 | 44,964 | 43,122 | 0.83% | 1,286 | 35 | City |
| New Smyrna Beach | 11 | 33,815 | 30,254 | 2.02% | 894 | 38 | City |
| New Smyrna Beach | 12 | 33,815 | 30,458 | 2.02% | 894 | 38 | City |
| Edgewater | 13 | 24,631 | 23,113 | 1.22% | 993 | 25 | City |
| Edgewater | 14 | 24,631 | 23,146 | 1.22% | 993 | 25 | City |
| DeBary | 15 | 23,775 | 22,185 | 1.31% | 1,255 | 19 | City |
| DeBary | 16 | 23,775 | 22,239 | 1.31% | 1,255 | 19 | City |
| Orange City | 17 | 15,877 | 12,706 | 4.06% | 2,031 | 8 | City |
| Orange City | 18 | 15,877 | 12,777 | 4.06% | 2,031 | 8 | City |
| South Daytona | 19 | 14,138 | 12,851 | 1.67% | 3,820 | 4 | City |
| South Daytona | 20 | 14,138 | 12,976 | 1.67% | 3,820 | 4 | City |
| Holly Hill | 21 | 13,234 | 12,969 | 0.39% | 3,340 | 4 | City |
| Holly Hill | 22 | 13,234 | 12,976 | 0.39% | 3,340 | 4 | City |
| Ormond-by-the-Sea | 23 | 6,484 | 6,999 | -0.93% | 3,216 | 2 | CDP |
| Daytona Beach Shores | 24 | 5,248 | 5,117 | 0.48% | 6,369 | 1 | City |
| Daytona Beach Shores | 25 | 5,248 | 5,120 | 0.48% | 6,369 | 1 | City |
| Samsula-Spruce Creek | 26 | 5,160 | 5,016 | 0.16% | 307 | 17 | CDP |
| Ponce Inlet | 27 | 3,513 | 3,367 | 0.8% | 817 | 4 | Town |
| Ponce Inlet | 28 | 3,513 | 3,372 | 0.8% | 817 | 4 | Town |
| Lake Helen | 29 | 3,024 | 2,842 | 1.2% | 664 | 5 | City |
| Lake Helen | 30 | 3,024 | 2,842 | 1.2% | 664 | 5 | City |
| De Leon Springs | 31 | 2,778 | 2,982 | 0.4% | 1,030 | 3 | CDP |
| Oak Hill | 32 | 2,209 | 2,014 | 1.7% | 315 | 7 | City |
| Oak Hill | 33 | 2,209 | 2,021 | 1.7% | 315 | 7 | City |
| Pierson | 34 | 1,568 | 1,540 | 0.32% | 154 | 10 | Town |
| Pierson | 35 | 1,568 | 1,541 | 0.32% | 154 | 10 | Town |
| Seville | 36 | 1,484 | 917 | 4.07% | 209 | 7 | CDP |
| Glencoe | 37 | 1,127 | 1,439 | -7.92% | 332 | 3 | CDP |
Deltona is the largest city in Volusia County, with a population of 102,129.
Glencoe is the smallest city in Volusia County, with a population of 1,127.
There are 37 cities in Volusia County, Florida.