23 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Clintonia | 1 | 7,213 | 7,314 | -0.14% | 240 | 30 | Township |
| Clinton | 2 | 6,858 | 6,972 | -0.33% | 1,935 | 4 | City |
| Santa Anna | 3 | 2,274 | 2,288 | 0% | 79 | 29 | Township |
| Clinton | 4 | 2,037 | 1,805 | 6.37% | 58 | 35 | Township |
| Farmer City | 5 | 1,814 | 1,828 | -0.17% | 757 | 2 | City |
| Texas | 6 | 1,243 | 1,255 | -0.08% | 35 | 36 | Township |
| Wapella | 7 | 899 | 913 | -0.11% | 31 | 29 | Township |
| McLean | 8 | 734 | 741 | -0.27% | 624 | 1 | Village |
| Tunbridge | 9 | 727 | 728 | 0% | 19 | 37 | Township |
| Waynesville | 10 | 630 | 637 | -0.16% | 26 | 24 | Township |
| Wapella | 11 | 501 | 511 | -0.4% | 909 | 1 | Village |
| Creek | 12 | 483 | 485 | -0.21% | 14 | 35 | Township |
| Nixon | 13 | 476 | 480 | 0% | 17 | 28 | Township |
| De Witt | 14 | 428 | 424 | 0.47% | 13 | 32 | Township |
| Waynesville | 15 | 375 | 379 | -0.27% | 1,176 | 0 | Village |
| Barnett | 16 | 364 | 366 | 0% | 10 | 37 | Township |
| Weldon | 17 | 358 | 361 | -0.28% | 1,341 | 0 | Village |
| Kenney | 18 | 306 | 305 | 0% | 1,085 | 0 | Village |
| Harp | 19 | 295 | 296 | 0% | 9 | 31 | Township |
| De Witt | 20 | 165 | 162 | 0% | 696 | 0 | Village |
| Lane | 21 | 161 | 164 | 7.33% | 1,309 | 0 | CDP |
| Rutledge | 22 | 151 | 152 | 0% | 6 | 24 | Township |
| Wilson | 23 | 124 | 126 | 0% | 5 | 24 | Township |
Clintonia is the largest city in De Witt County, with a population of 7,213.
Wilson is the smallest city in De Witt County, with a population of 124.
There are 23 cities in De Witt County, Illinois.