39 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Center | 1 | 1,702 | 1,669 | 0.71% | 27 | 63 | Township |
| Center | 2 | 1,702 | 1,661 | 0.71% | 27 | 63 | Township |
| Iowa | 3 | 1,463 | 1,445 | 0.48% | 18 | 84 | Township |
| Iowa | 4 | 1,463 | 1,439 | 0.48% | 18 | 84 | Township |
| Wathena | 5 | 1,263 | 1,251 | 0.24% | 539 | 2 | City |
| Wathena | 6 | 1,263 | 1,245 | 0.24% | 539 | 2 | City |
| Elwood | 7 | 1,142 | 1,119 | 0.44% | 392 | 3 | City |
| Elwood | 8 | 1,142 | 1,114 | 0.44% | 392 | 3 | City |
| Troy | 9 | 986 | 968 | 0.41% | 1,011 | 1 | City |
| Troy | 10 | 986 | 963 | 0.41% | 1,011 | 1 | City |
| Highland | 11 | 934 | 924 | 0.21% | 1,749 | 1 | City |
| Highland | 12 | 934 | 921 | 0.21% | 1,749 | 1 | City |
| Highland | 13 | 375 | 376 | 0.27% | 11 | 35 | Township |
| Highland | 14 | 375 | 375 | 0.27% | 11 | 35 | Township |
| Wolf River | 15 | 343 | 339 | 0.59% | 6 | 56 | Township |
| Wolf River | 16 | 343 | 338 | 0.59% | 6 | 56 | Township |
| Union | 17 | 270 | 270 | 0% | 7 | 36 | Township |
| Union | 18 | 270 | 268 | 0% | 7 | 36 | Township |
| Elwood | 19 | 201 | 209 | 0% | 2 | 100 | Township |
| Elwood | 20 | 201 | 208 | 0% | 2 | 100 | Township |
| Wayne | 21 | 192 | 190 | 0.52% | 5 | 37 | Township |
| Wayne | 22 | 192 | 189 | 0.52% | 5 | 37 | Township |
| Troy | 23 | 134 | 135 | 0% | 4 | 36 | Township |
| Troy | 24 | 134 | 135 | 0% | 4 | 36 | Township |
| Bendena | 25 | 134 | 126 | 0.75% | 88 | 2 | CDP |
| Denton | 26 | 133 | 132 | 0% | 917 | 0 | City |
| Denton | 27 | 133 | 131 | 0% | 917 | 0 | City |
| White Cloud | 28 | 115 | 114 | 0% | 159 | 1 | City |
| White Cloud | 29 | 115 | 113 | 0% | 159 | 1 | City |
| Highland | 30 | 82 | 84 | 0% | 2 | 35 | Township |
| Highland | 31 | 82 | 84 | 0% | 2 | 35 | Township |
| Severance | 32 | 79 | 78 | 0% | 564 | 0 | City |
| Severance | 33 | 79 | 78 | 0% | 564 | 0 | City |
| Highland | 34 | 53 | 54 | 0% | 1 | 36 | Township |
| Highland | 35 | 53 | 54 | 0% | 1 | 36 | Township |
| Highland | 36 | 47 | 47 | 0% | 1 | 36 | Township |
| Highland | 37 | 47 | 47 | 0% | 1 | 36 | Township |
| Highland | 38 | 19 | 19 | 0% | 1 | 36 | Township |
| Highland | 39 | 19 | 19 | 0% | 1 | 36 | Township |
Center is the largest city in Doniphan County, with a population of 1,702.
Highland is the smallest city in Doniphan County, with a population of 19.
There are 39 cities in Doniphan County, Kansas.