16 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Chanute | 1 | 8,284 | 8,744 | -1.1% | 1,167 | 7.10 | City |
| Erie | 2 | 1,217 | 1,272 | -0.81% | 26 | 47.34 | Township |
| Erie | 3 | 998 | 1,050 | -0.99% | 788 | 1.27 | City |
| Tioga | 4 | 795 | 850 | -1.49% | 19 | 41.29 | Township |
| St. Paul | 5 | 595 | 614 | -0.67% | 527 | 1.13 | City |
| Shiloh | 6 | 539 | 566 | -1.1% | 11 | 48.35 | Township |
| Canville | 7 | 512 | 544 | -1.35% | 11 | 47.31 | Township |
| Thayer | 8 | 417 | 437 | -0.95% | 765 | 0.55 | City |
| Big Creek | 9 | 415 | 446 | -1.66% | 9 | 47.92 | Township |
| Ladore | 10 | 359 | 377 | -1.1% | 8 | 46.84 | Township |
| Grant | 11 | 317 | 334 | -0.94% | 7 | 48.10 | Township |
| Walnut Grove | 12 | 303 | 319 | -0.98% | 6 | 48.21 | Township |
| Galesburg | 13 | 199 | 210 | -1% | 6 | 35.49 | Township |
| Galesburg | 14 | 137 | 146 | -1.44% | 801 | 0.17 | City |
| Erie | 15 | 115 | 105 | 2.68% | 3 | 35.30 | Township |
| Stark | 16 | 64 | 68 | -1.54% | 390 | 0.16 | City |
Chanute is the largest city in Neosho County, with a population of 8,284.
Stark is the smallest city in Neosho County, with a population of 64.
There are 16 cities in Neosho County, Kansas.