52 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Osborne | 1 | 1,262 | 1,325 | -0.94% | 820 | 2 | City |
| Osborne | 2 | 1,262 | 1,322 | -0.94% | 820 | 2 | City |
| Ross | 3 | 829 | 887 | -2.47% | 23 | 35 | Township |
| Ross | 4 | 829 | 886 | -2.47% | 23 | 35 | Township |
| Downs | 5 | 764 | 804 | -1.04% | 682 | 1 | City |
| Downs | 6 | 764 | 803 | -1.04% | 682 | 1 | City |
| Natoma | 7 | 301 | 326 | -3.22% | 8 | 36 | Township |
| Natoma | 8 | 301 | 326 | -3.22% | 8 | 36 | Township |
| Natoma | 9 | 286 | 304 | -1.38% | 678 | 0 | City |
| Natoma | 10 | 286 | 304 | -1.38% | 678 | 0 | City |
| Osborne | 11 | 218 | 220 | -0.91% | 6 | 34 | Township |
| Osborne | 12 | 218 | 220 | -0.91% | 6 | 34 | Township |
| Bethany | 13 | 142 | 150 | -1.39% | 4 | 36 | Township |
| Bethany | 14 | 142 | 150 | -1.39% | 4 | 36 | Township |
| Downs | 15 | 140 | 141 | -0.71% | 4 | 35 | Township |
| Downs | 16 | 140 | 141 | -0.71% | 4 | 35 | Township |
| Sumner | 17 | 138 | 148 | -2.13% | 3 | 54 | Township |
| Sumner | 18 | 138 | 148 | -2.13% | 3 | 54 | Township |
| Penn | 19 | 123 | 135 | -3.91% | 3 | 46 | Township |
| Penn | 20 | 123 | 135 | -3.91% | 3 | 46 | Township |
| Alton | 21 | 91 | 98 | -2.15% | 294 | 0 | City |
| Alton | 22 | 91 | 98 | -2.15% | 294 | 0 | City |
| Portis | 23 | 83 | 88 | -1.19% | 318 | 0 | City |
| Portis | 24 | 83 | 88 | -1.19% | 318 | 0 | City |
| Tilden | 25 | 68 | 73 | -2.86% | 2 | 41 | Township |
| Tilden | 26 | 68 | 73 | -2.86% | 2 | 41 | Township |
| Bloom | 27 | 63 | 66 | -1.56% | 1 | 54 | Township |
| Bloom | 28 | 63 | 66 | -1.56% | 1 | 54 | Township |
| Corinth | 29 | 44 | 47 | -2.22% | 1 | 35 | Township |
| Corinth | 30 | 44 | 47 | -2.22% | 1 | 35 | Township |
| Valley | 31 | 34 | 35 | 0% | 1 | 36 | Township |
| Valley | 32 | 34 | 35 | 0% | 1 | 36 | Township |
| Delhi | 33 | 29 | 30 | 0% | 1 | 54 | Township |
| Delhi | 34 | 29 | 30 | 0% | 1 | 54 | Township |
| Hawkeye | 35 | 27 | 29 | -3.57% | 1 | 36 | Township |
| Hawkeye | 36 | 27 | 29 | -3.57% | 1 | 36 | Township |
| Jackson | 37 | 27 | 29 | -3.57% | 1 | 36 | Township |
| Jackson | 38 | 27 | 29 | -3.57% | 1 | 36 | Township |
| Round Mound | 39 | 26 | 28 | -3.7% | 1 | 36 | Township |
| Round Mound | 40 | 26 | 28 | -3.7% | 1 | 36 | Township |
| Grant | 41 | 22 | 24 | -4.35% | 1 | 35 | Township |
| Grant | 42 | 22 | 24 | -4.35% | 1 | 35 | Township |
| Mount Ayr | 43 | 19 | 21 | -5% | 1 | 36 | Township |
| Mount Ayr | 44 | 19 | 21 | -5% | 1 | 36 | Township |
| Hancock | 45 | 16 | 18 | -5.88% | 0.00 | 36 | Township |
| Hancock | 46 | 16 | 18 | -5.88% | 0.00 | 36 | Township |
| Victor | 47 | 12 | 12 | 0% | 0.00 | 36 | Township |
| Victor | 48 | 12 | 12 | 0% | 0.00 | 36 | Township |
| Covert | 49 | 10 | 10 | 0% | 0.00 | 36 | Township |
| Covert | 50 | 10 | 10 | 0% | 0.00 | 36 | Township |
| Kill Creek | 51 | 9 | 9 | 0% | 0.00 | 36 | Township |
| Kill Creek | 52 | 9 | 9 | 0% | 0.00 | 36 | Township |
Osborne is the largest city in Osborne County, with a population of 1,262.
Kill Creek is the smallest city in Osborne County, with a population of 9.
There are 52 cities in Osborne County, Kansas.