3 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Nicholasville | 1 | 34,039 | 31,131 | 1.74% | 2,269 | 15 | City |
| Wilmore | 2 | 6,333 | 6,006 | 1.04% | 1,272 | 5 | City |
| Keene | 3 | 107 | 99 | 0.94% | 870 | 0 | City |
Nicholasville is the largest city in Jessamine County, with a population of 34,039.
Keene is the smallest city in Jessamine County, with a population of 107.
There are 3 cities in Jessamine County, Kentucky.