55 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Barnstable Town | 1 | 50,070 | 48,872 | 0.48% | 835 | 60 | City |
| Barnstable Town | 2 | 49,704 | 48,872 | -0.25% | 829 | 60 | City |
| Falmouth | 3 | 33,247 | 32,541 | 0.06% | 755 | 44 | Town |
| Yarmouth | 4 | 25,179 | 24,990 | -0.12% | 1,042 | 24 | Town |
| Bourne | 5 | 20,544 | 19,604 | 0.18% | 505 | 41 | Town |
| Sandwich | 6 | 20,459 | 20,254 | -0.23% | 477 | 43 | Town |
| Mashpee | 7 | 15,479 | 15,068 | -0.02% | 662 | 23 | Town |
| Dennis | 8 | 14,762 | 14,676 | -0.67% | 717 | 21 | Town |
| Harwich | 9 | 13,589 | 13,430 | -0.23% | 651 | 21 | Town |
| South Yarmouth | 10 | 11,983 | 10,598 | 0.49% | 1,723 | 7 | CDP |
| Brewster | 11 | 10,466 | 10,318 | 0.18% | 456 | 23 | Town |
| Chatham | 12 | 6,713 | 6,593 | 0.03% | 415 | 16 | Town |
| East Falmouth | 13 | 6,688 | 6,163 | 0.65% | 1,230 | 5 | CDP |
| Orleans | 14 | 6,552 | 6,306 | 1.03% | 463 | 14 | Town |
| Yarmouth Port | 15 | 6,134 | 5,202 | 0.89% | 1,019 | 6 | CDP |
| West Yarmouth | 16 | 6,120 | 6,388 | 0.13% | 915 | 7 | CDP |
| East Harwich | 17 | 5,898 | 4,756 | 1.17% | 735 | 8 | CDP |
| Eastham | 18 | 5,823 | 5,746 | -0.02% | 417 | 14 | Town |
| Northwest Harwich | 19 | 4,113 | 4,123 | 0.32% | 515 | 8 | CDP |
| Sagamore | 20 | 4,052 | 3,792 | 0.72% | 1,217 | 3 | CDP |
| East Dennis | 21 | 3,951 | 3,068 | 2.07% | 817 | 5 | CDP |
| Falmouth | 22 | 3,904 | 3,751 | 0.18% | 1,870 | 2 | CDP |
| Forestdale | 23 | 3,865 | 3,744 | -0.41% | 1,013 | 4 | CDP |
| East Sandwich | 24 | 3,749 | 3,628 | -0.35% | 499 | 8 | CDP |
| Provincetown | 25 | 3,731 | 3,658 | 0.05% | 386 | 10 | Town |
| Wellfleet | 26 | 3,648 | 3,565 | 0.08% | 184 | 20 | Town |
| Provincetown | 27 | 3,631 | 2,730 | 1.85% | 2,032 | 2 | CDP |
| Dennis Port | 28 | 3,334 | 3,523 | 0.33% | 1,097 | 3 | CDP |
| Monument Beach | 29 | 3,082 | 3,173 | 0.62% | 1,194 | 3 | CDP |
| South Dennis | 30 | 2,966 | 2,895 | -1.49% | 652 | 5 | CDP |
| Sandwich | 31 | 2,945 | 2,928 | -0.03% | 813 | 4 | CDP |
| West Dennis | 32 | 2,855 | 2,479 | 1.46% | 875 | 3 | CDP |
| Buzzards Bay | 33 | 2,777 | 3,208 | -2.53% | 1,449 | 2 | CDP |
| Pocasset | 34 | 2,571 | 2,596 | -0.73% | 695 | 4 | CDP |
| Truro | 35 | 2,485 | 2,439 | 0.04% | 119 | 21 | Town |
| Harwich Port | 36 | 2,404 | 1,906 | 2.17% | 913 | 3 | CDP |
| North Falmouth | 37 | 2,126 | 2,572 | -2.92% | 546 | 4 | CDP |
| North Eastham | 38 | 2,012 | 1,741 | 0.7% | 584 | 3 | CDP |
| Bourne | 39 | 1,873 | 1,377 | 1.63% | 1,099 | 2 | CDP |
| West Falmouth | 40 | 1,807 | 1,836 | 0.28% | 586 | 3 | CDP |
| Dennis | 41 | 1,771 | 1,962 | -2.32% | 361 | 5 | CDP |
| Chatham | 42 | 1,691 | 1,550 | 1.08% | 641 | 3 | CDP |
| West Chatham | 43 | 1,689 | 1,224 | 1.14% | 578 | 3 | CDP |
| Orleans | 44 | 1,676 | 1,519 | 0.24% | 722 | 2 | CDP |
| Brewster | 45 | 1,674 | 1,347 | -1.3% | 430 | 4 | CDP |
| Teaticket | 46 | 1,528 | 1,608 | -0.71% | 1,450 | 1 | CDP |
| Mashpee Neck | 47 | 1,338 | 1,403 | 1.75% | 1,057 | 1 | CDP |
| Harwich Center | 48 | 1,324 | 1,383 | -2.36% | 589 | 2 | CDP |
| Woods Hole | 49 | 1,045 | 933 | 1.75% | 489 | 2 | CDP |
| New Seabury | 50 | 743 | 595 | 0.13% | 308 | 2 | CDP |
| Seabrook | 51 | 630 | 520 | 1.94% | 1,790 | 0 | CDP |
| Popponesset | 52 | 403 | 202 | 3.33% | 1,348 | 0 | CDP |
| Seconsett Island | 53 | 146 | 37 | 2.1% | 1,417 | 0 | CDP |
| Monomoscoy Island | 54 | 117 | 114 | -1.68% | 504 | 0 | CDP |
| Popponesset Island | 55 | 87 | 35 | 6.1% | 1,208 | 0 | CDP |
Barnstable Town is the largest city in Barnstable County, with a population of 50,070.
Popponesset Island is the smallest city in Barnstable County, with a population of 87.
There are 55 cities in Barnstable County, Massachusetts.