33 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Caro | 1 | 4,247 | 4,319 | -0.35% | 1,443 | 3 | City |
| Millington | 2 | 4,172 | 4,235 | -0.22% | 117 | 36 | Township |
| Vassar | 3 | 3,869 | 3,883 | -0.03% | 110 | 35 | Township |
| Elkland | 4 | 3,481 | 3,531 | -0.2% | 99 | 35 | Township |
| Arbela | 5 | 2,761 | 2,807 | -0.32% | 83 | 33 | Township |
| Denmark | 6 | 2,704 | 2,748 | -0.26% | 77 | 35 | Township |
| Vassar | 7 | 2,683 | 2,727 | -0.33% | 1,249 | 2 | City |
| Cass City | 8 | 2,477 | 2,512 | -0.28% | 1,403 | 2 | Village |
| Indianfields | 9 | 2,454 | 2,474 | -0.16% | 78 | 31 | Township |
| Watertown | 10 | 2,071 | 2,095 | -0.24% | 64 | 33 | Township |
| Almer | 11 | 1,981 | 1,987 | 0% | 59 | 34 | Township |
| Tuscola | 12 | 1,933 | 1,977 | -0.46% | 59 | 33 | Township |
| Dayton | 13 | 1,899 | 1,922 | -0.21% | 53 | 36 | Township |
| Juniata | 14 | 1,550 | 1,560 | -0.06% | 44 | 35 | Township |
| Fairgrove | 15 | 1,529 | 1,552 | -0.13% | 43 | 35 | Township |
| Koylton | 16 | 1,468 | 1,481 | -0.2% | 42 | 35 | Township |
| Kingston | 17 | 1,451 | 1,475 | -0.34% | 41 | 35 | Township |
| Novesta | 18 | 1,428 | 1,442 | -0.21% | 40 | 35 | Township |
| Akron | 19 | 1,353 | 1,375 | -0.22% | 26 | 53 | Township |
| Ellington | 20 | 1,318 | 1,331 | -0.08% | 37 | 35 | Township |
| Reese | 21 | 1,256 | 1,280 | -0.4% | 930 | 1 | Village |
| Columbia | 22 | 1,206 | 1,229 | -0.25% | 34 | 36 | Township |
| Fostoria | 23 | 1,105 | 752 | 2.5% | 276 | 4 | CDP |
| Elmwood | 24 | 1,048 | 1,062 | -0.1% | 30 | 35 | Township |
| Millington | 25 | 1,010 | 1,029 | -0.39% | 742 | 1 | Village |
| Mayville | 26 | 902 | 921 | -0.44% | 838 | 1 | Village |
| Gilford | 27 | 724 | 740 | -0.41% | 21 | 35 | Township |
| Wisner | 28 | 594 | 604 | -0.34% | 31 | 19 | Township |
| Fairgrove | 29 | 520 | 532 | -0.57% | 464 | 1 | Village |
| Unionville | 30 | 482 | 492 | -0.41% | 513 | 1 | Village |
| Kingston | 31 | 391 | 400 | -0.51% | 384 | 1 | Village |
| Akron | 32 | 366 | 374 | -0.54% | 389 | 1 | Village |
| Gagetown | 33 | 316 | 323 | -0.63% | 322 | 1 | Village |
Caro is the largest city in Tuscola County, with a population of 4,247.
Gagetown is the smallest city in Tuscola County, with a population of 316.
There are 33 cities in Tuscola County, Michigan.