4 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Mayersville | 1 | 472 | 431 | 1.72% | 429 | 1 | Town |
| Grace | 2 | 284 | 130 | 7.17% | 59 | 5 | CDP |
| Eagle Bend | 3 | 253 | 504 | 7.2% | 151 | 2 | CDP |
| Valley Park | 4 | 150 | 258 | 7.14% | 33 | 5 | CDP |
Mayersville is the largest city in Issaquena County, with a population of 472.
Valley Park is the smallest city in Issaquena County, with a population of 150.
There are 4 cities in Issaquena County, Mississippi.