9 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Poplar Bluff | 1 | 16,150 | 16,309 | -0.17% | 1,229 | 13 | City |
| Poplar Bluff | 2 | 16,150 | 16,283 | -0.17% | 1,229 | 13 | City |
| Qulin | 3 | 467 | 470 | -0.21% | 1,035 | 0 | City |
| Qulin | 4 | 467 | 469 | -0.21% | 1,035 | 0 | City |
| Neelyville | 5 | 310 | 315 | -0.32% | 270 | 1 | City |
| Neelyville | 6 | 310 | 315 | -0.32% | 270 | 1 | City |
| Fisk | 7 | 308 | 312 | -0.32% | 951 | 0 | City |
| Fisk | 8 | 308 | 311 | -0.32% | 951 | 0 | City |
| Broseley | 9 | 69 | 168 | 7.81% | 133 | 1 | CDP |
Poplar Bluff is the largest city in Butler County, with a population of 16,150.
Broseley is the smallest city in Butler County, with a population of 69.
There are 9 cities in Butler County, Missouri.