19 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Gallatin | 1 | 1,873 | 1,825 | 0.54% | 682 | 3 | City |
| Jamesport | 2 | 1,122 | 1,092 | 0.81% | 33 | 34 | Township |
| Colfax | 3 | 668 | 642 | 1.21% | 19 | 36 | Township |
| Jamesport | 4 | 571 | 560 | 0.35% | 1,018 | 1 | City |
| Jefferson | 5 | 477 | 464 | 0.85% | 13 | 36 | Township |
| Lake Viking | 6 | 383 | 355 | -1.79% | 49 | 8 | CDP |
| Pattonsburg | 7 | 343 | 333 | 0.59% | 566 | 1 | City |
| Grand River | 8 | 304 | 294 | 1% | 6 | 53 | Township |
| Marion | 9 | 267 | 257 | 1.14% | 6 | 46 | Township |
| Winston | 10 | 239 | 229 | 0.84% | 776 | 0 | Village |
| Monroe | 11 | 190 | 183 | 1.06% | 5 | 36 | Township |
| Altamont | 12 | 173 | 167 | 0.58% | 676 | 0 | Village |
| Harrison | 13 | 157 | 153 | 0.64% | 7 | 22 | Township |
| Coffey | 14 | 153 | 150 | 0.66% | 736 | 0 | City |
| McFall | 15 | 120 | 119 | 0% | 391 | 0 | City |
| Jameson | 16 | 71 | 70 | 0% | 317 | 0 | Town |
| Avalon | 17 | 59 | 51 | 7.27% | 79 | 1 | CDP |
| Brimson | 18 | 47 | 47 | 0% | 540 | 0 | Village |
| Lock Springs | 19 | 39 | 38 | 0% | 315 | 0 | Village |
Gallatin is the largest city in Daviess County, with a population of 1,873.
Lock Springs is the smallest city in Daviess County, with a population of 39.
There are 19 cities in Daviess County, Missouri.