18 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Springfield | 1 | 170,955 | 169,149 | 0.21% | 2,055 | 83 | City |
| Springfield | 2 | 170,955 | 169,158 | 0.21% | 2,055 | 83 | City |
| Republic | 3 | 22,014 | 18,763 | 2.91% | 1,374 | 16 | City |
| Republic | 4 | 22,014 | 18,903 | 2.91% | 1,374 | 16 | City |
| Willard | 5 | 6,697 | 6,346 | 0.98% | 1,107 | 6 | City |
| Willard | 6 | 6,697 | 6,372 | 0.98% | 1,107 | 6 | City |
| Strafford | 7 | 2,859 | 2,557 | 2.07% | 869 | 3 | City |
| Strafford | 8 | 2,859 | 2,567 | 2.07% | 869 | 3 | City |
| Fair Grove | 9 | 1,634 | 1,586 | 0.55% | 513 | 3 | City |
| Fair Grove | 10 | 1,634 | 1,589 | 0.55% | 513 | 3 | City |
| Ash Grove | 11 | 1,524 | 1,517 | 0.07% | 1,148 | 1 | City |
| Ash Grove | 12 | 1,524 | 1,517 | 0.07% | 1,148 | 1 | City |
| Pleasant Hope | 13 | 697 | 669 | 0.72% | 458 | 2 | City |
| Pleasant Hope | 14 | 697 | 671 | 0.72% | 458 | 2 | City |
| Walnut Grove | 15 | 640 | 648 | -0.31% | 1,031 | 1 | City |
| Walnut Grove | 16 | 640 | 648 | -0.31% | 1,031 | 1 | City |
| Springfield | 17 | 313 | 303 | 0.64% | 10 | 33 | Township |
| Springfield | 18 | 313 | 303 | 0.64% | 10 | 33 | Township |
Springfield is the largest city in Greene County, with a population of 170,955.
Springfield is the smallest city in Greene County, with a population of 313.
There are 18 cities in Greene County, Missouri.