5 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Mound City | 1 | 1,021 | 998 | 0.49% | 819 | 1.25 | City |
| Oregon | 2 | 840 | 825 | 0.36% | 905 | 0.93 | City |
| Maitland | 3 | 281 | 273 | 0.72% | 901 | 0.31 | City |
| Forest City | 4 | 248 | 242 | 0.4% | 253 | 0.98 | City |
| Craig | 5 | 104 | 103 | 0% | 400 | 0.26 | City |
Mound City is the largest city in Holt County, with a population of 1,021.
Craig is the smallest city in Holt County, with a population of 104.
There are 5 cities in Holt County, Missouri.