10 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Mound City | 1 | 1,007 | 1,005 | 0.2% | 808 | 1 | City |
| Mound City | 2 | 1,007 | 994 | 0.2% | 808 | 1 | City |
| Oregon | 3 | 833 | 834 | 0.12% | 898 | 1 | City |
| Oregon | 4 | 833 | 826 | 0.12% | 898 | 1 | City |
| Maitland | 5 | 281 | 278 | 0.36% | 901 | 0 | City |
| Maitland | 6 | 281 | 275 | 0.36% | 901 | 0 | City |
| Forest City | 7 | 248 | 247 | 0% | 253 | 1 | City |
| Forest City | 8 | 248 | 245 | 0% | 253 | 1 | City |
| Craig | 9 | 104 | 104 | 0% | 400 | 0 | City |
| Craig | 10 | 104 | 103 | 0% | 400 | 0 | City |
Mound City is the largest city in Holt County, with a population of 1,007.
Craig is the smallest city in Holt County, with a population of 104.
There are 10 cities in Holt County, Missouri.