6 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Fredericktown | 1 | 4,617 | 4,435 | 0.79% | 1,068 | 4 | City |
| Junction City | 2 | 286 | 285 | 0% | 799 | 0 | Village |
| Cobalt | 3 | 263 | 261 | 0% | 1,765 | 0 | Village |
| Mine La Motte | 4 | 208 | 387 | -4.15% | 64 | 3 | CDP |
| Marquand | 5 | 190 | 188 | 0% | 798 | 0 | City |
| Cherokee Pass | 6 | 186 | 261 | -2.11% | 89 | 2 | CDP |
Fredericktown is the largest city in Madison County, with a population of 4,617.
Cherokee Pass is the smallest city in Madison County, with a population of 186.
There are 6 cities in Madison County, Missouri.