9 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Charleston | 1 | 4,397 | 4,611 | -0.97% | 917 | 5 | City |
| East Prairie | 2 | 2,825 | 2,937 | -0.77% | 2,260 | 1 | City |
| Miner | 3 | 893 | 910 | -0.33% | 194 | 5 | City |
| Bertrand | 4 | 691 | 716 | -0.72% | 839 | 1 | City |
| Wyatt | 5 | 209 | 221 | -0.95% | 178 | 1 | City |
| Whiting | 6 | 190 | 134 | 7.34% | 728 | 0 | CDP |
| Anniston | 7 | 176 | 180 | -0.56% | 450 | 0 | Town |
| Diehlstadt | 8 | 155 | 159 | -0.64% | 2,095 | 0 | Village |
| Wilson City | 9 | 73 | 77 | -1.35% | 973 | 0 | Village |
Charleston is the largest city in Mississippi County, with a population of 4,397.
Wilson City is the smallest city in Mississippi County, with a population of 73.
There are 9 cities in Mississippi County, Missouri.