9 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Monroe City | 1 | 2,653 | 2,657 | -0.04% | 879 | 3 | City |
| Paris | 2 | 1,152 | 1,154 | 0% | 912 | 1 | City |
| Madison | 3 | 598 | 603 | -0.5% | 17 | 35 | Township |
| Madison | 4 | 520 | 520 | 0% | 1,161 | 0 | City |
| Madison | 5 | 419 | 421 | -0.48% | 11 | 38 | Township |
| Madison | 6 | 247 | 247 | 0.41% | 6 | 41 | Township |
| Holliday | 7 | 114 | 114 | 0% | 437 | 0 | Village |
| Middle Grove | 8 | 60 | 37 | 7.14% | 108 | 1 | CDP |
| Stoutsville | 9 | 37 | 37 | 0% | 45 | 1 | Village |
Monroe City is the largest city in Monroe County, with a population of 2,653.
Stoutsville is the smallest city in Monroe County, with a population of 37.
There are 9 cities in Monroe County, Missouri.