25 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Maryville | 1 | 10,102 | 10,619 | -1.01% | 1,554 | 6 | City |
| Nodaway | 2 | 788 | 861 | -2.35% | 17 | 47 | Township |
| Jefferson | 3 | 616 | 659 | -1.6% | 14 | 44 | Township |
| Hopkins | 4 | 592 | 641 | -1.99% | 15 | 39 | Township |
| Burlington Junction | 5 | 483 | 520 | -1.43% | 483 | 1 | City |
| White Cloud | 6 | 482 | 524 | -2.03% | 9 | 53 | Township |
| Grant | 7 | 470 | 518 | -2.69% | 10 | 46 | Township |
| Hopkins | 8 | 435 | 465 | -1.36% | 600 | 1 | City |
| Ravenwood | 9 | 408 | 437 | -1.45% | 1,338 | 0 | Town |
| Hughes | 10 | 383 | 417 | -2.3% | 6 | 64 | Township |
| Atchison | 11 | 367 | 400 | -2.13% | 7 | 56 | Township |
| Monroe | 12 | 360 | 394 | -2.17% | 8 | 44 | Township |
| Conception | 13 | 280 | 291 | 1.82% | 373 | 1 | CDP |
| Skidmore | 14 | 227 | 244 | -1.3% | 682 | 0 | City |
| Green | 15 | 208 | 225 | -1.89% | 3 | 67 | Township |
| Barnard | 16 | 186 | 200 | -1.59% | 1,224 | 0 | City |
| Conception Junction | 17 | 166 | 178 | -1.19% | 525 | 0 | Town |
| Clearmont | 18 | 147 | 157 | -1.34% | 896 | 0 | City |
| Graham | 19 | 142 | 152 | -1.39% | 534 | 0 | Town |
| Pickering | 20 | 141 | 151 | -1.4% | 825 | 0 | City |
| Parnell | 21 | 129 | 139 | -1.53% | 464 | 0 | City |
| Elmo | 22 | 107 | 116 | -1.83% | 493 | 0 | City |
| Guilford | 23 | 58 | 62 | -1.69% | 806 | 0 | Town |
| Clyde | 24 | 52 | 56 | -1.89% | 308 | 0 | Village |
| Arkoe | 25 | 51 | 55 | -1.92% | 402 | 0 | Town |
Maryville is the largest city in Nodaway County, with a population of 10,102.
Arkoe is the smallest city in Nodaway County, with a population of 51.
There are 25 cities in Nodaway County, Missouri.