53 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Union | 1 | 54,144 | 56,140 | -0.28% | 1,528 | 35 | Town |
| Union | 2 | 54,144 | 55,953 | -0.28% | 1,528 | 35 | Town |
| Binghamton | 3 | 46,521 | 47,945 | -0.54% | 4,439 | 10 | City |
| Binghamton | 4 | 46,521 | 47,779 | -0.54% | 4,439 | 10 | City |
| Vestal | 5 | 32,072 | 29,315 | 0.71% | 621 | 52 | Town |
| Vestal | 6 | 32,072 | 29,330 | 0.71% | 621 | 52 | Town |
| Johnson City | 7 | 14,590 | 15,211 | -0.73% | 3,205 | 5 | Village |
| Johnson City | 8 | 14,590 | 15,128 | -0.73% | 3,205 | 5 | Village |
| Endicott | 9 | 13,088 | 13,675 | -0.84% | 4,098 | 3 | Village |
| Endicott | 10 | 13,088 | 13,643 | -0.84% | 4,098 | 3 | Village |
| Endwell | 11 | 11,921 | 11,396 | 0.27% | 3,184 | 4 | CDP |
| Chenango | 12 | 10,635 | 10,973 | -0.16% | 314 | 34 | Town |
| Chenango | 13 | 10,635 | 10,947 | -0.16% | 314 | 34 | Town |
| Binghamton University | 14 | 6,462 | 6,162 | 0.29% | 8,338 | 1 | CDP |
| Fenton | 15 | 6,242 | 6,429 | 0.02% | 190 | 33 | Town |
| Fenton | 16 | 6,242 | 6,410 | 0.02% | 190 | 33 | Town |
| Windsor | 17 | 5,627 | 5,798 | -0.12% | 61 | 92 | Town |
| Windsor | 18 | 5,627 | 5,781 | -0.12% | 61 | 92 | Town |
| Kirkwood | 19 | 5,311 | 5,494 | -0.23% | 173 | 31 | Town |
| Kirkwood | 20 | 5,311 | 5,480 | -0.23% | 173 | 31 | Town |
| Maine | 21 | 5,022 | 5,176 | -0.14% | 110 | 46 | Town |
| Maine | 22 | 5,022 | 5,163 | -0.14% | 110 | 46 | Town |
| Dickinson | 23 | 4,953 | 5,100 | -0.16% | 1,039 | 5 | Town |
| Dickinson | 24 | 4,953 | 5,079 | -0.16% | 1,039 | 5 | Town |
| Conklin | 25 | 4,849 | 5,014 | -0.14% | 198 | 24 | Town |
| Conklin | 26 | 4,849 | 5,001 | -0.14% | 198 | 24 | Town |
| Colesville | 27 | 4,754 | 4,869 | -0.02% | 61 | 78 | Town |
| Colesville | 28 | 4,754 | 4,859 | -0.02% | 61 | 78 | Town |
| Binghamton | 29 | 4,467 | 4,612 | -0.25% | 177 | 25 | Town |
| Binghamton | 30 | 4,467 | 4,600 | -0.25% | 177 | 25 | Town |
| Chenango Bridge | 31 | 3,309 | 3,155 | 0.85% | 1,351 | 2 | CDP |
| Triangle | 32 | 2,732 | 2,811 | -0.04% | 72 | 38 | Town |
| Triangle | 33 | 2,732 | 2,804 | -0.04% | 72 | 38 | Town |
| Lisle | 34 | 2,640 | 2,692 | 0% | 56 | 47 | Town |
| Lisle | 35 | 2,640 | 2,685 | 0% | 56 | 47 | Town |
| Crest View Heights | 36 | 2,322 | 1,880 | 7.1% | 1,900 | 1 | CDP |
| Sanford | 37 | 2,181 | 2,236 | 0.05% | 24 | 90 | Town |
| Sanford | 38 | 2,181 | 2,230 | 0.05% | 24 | 90 | Town |
| Port Dickinson | 39 | 1,660 | 1,728 | -0.78% | 2,648 | 1 | Village |
| Port Dickinson | 40 | 1,660 | 1,724 | -0.78% | 2,648 | 1 | Village |
| Nanticoke | 41 | 1,554 | 1,574 | 0.52% | 64 | 24 | Town |
| Nanticoke | 42 | 1,554 | 1,570 | 0.52% | 64 | 24 | Town |
| Deposit | 43 | 1,403 | 1,455 | -0.71% | 1,111 | 1 | Village |
| Deposit | 44 | 1,403 | 1,452 | -0.71% | 1,111 | 1 | Village |
| Deposit | 45 | 1,383 | 1,424 | -0.79% | 32 | 43 | Town |
| Deposit | 46 | 1,383 | 1,422 | -0.79% | 32 | 43 | Town |
| Whitney Point | 47 | 891 | 928 | -0.78% | 854 | 1 | Village |
| Whitney Point | 48 | 891 | 926 | -0.78% | 854 | 1 | Village |
| Windsor | 49 | 860 | 896 | -0.81% | 783 | 1 | Village |
| Windsor | 50 | 860 | 894 | -0.81% | 783 | 1 | Village |
| Lisle | 51 | 323 | 337 | -0.92% | 345 | 1 | Village |
| Lisle | 52 | 323 | 336 | -0.92% | 345 | 1 | Village |
| Glen Aubrey | 53 | 153 | 258 | -12.57% | 155 | 1 | CDP |
Union is the largest city in Broome County, with a population of 54,144.
Glen Aubrey is the smallest city in Broome County, with a population of 153.
There are 53 cities in Broome County, New York.