61 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Buffalo | 1 | 276,381 | 277,558 | -0.09% | 6,845 | 40 | City |
| Amherst | 2 | 130,981 | 129,359 | 0.09% | 2,462 | 53 | Town |
| Cheektowaga | 3 | 88,488 | 89,748 | 0% | 3,005 | 29 | Town |
| Cheektowaga | 4 | 76,809 | 73,457 | 0.14% | 3,027 | 25 | CDP |
| Tonawanda Town | 5 | 71,512 | 56,829 | 7.14% | 4,134 | 17 | CDP |
| Tonawanda | 6 | 71,393 | 72,444 | -0.03% | 3,811 | 19 | Town |
| Hamburg | 7 | 61,097 | 60,052 | 0.8% | 1,479 | 41 | Town |
| West Seneca | 8 | 45,586 | 45,470 | 0.45% | 2,131 | 21 | Town |
| West Seneca | 9 | 45,556 | 45,272 | 0.12% | 2,130 | 21 | CDP |
| Lancaster | 10 | 45,466 | 45,039 | 0.36% | 1,206 | 38 | Town |
| Clarence | 11 | 33,359 | 32,948 | 0.28% | 623 | 54 | Town |
| Orchard Park | 12 | 29,970 | 29,668 | 0.28% | 780 | 38 | Town |
| Grand Island | 13 | 21,651 | 21,448 | 0.4% | 766 | 28 | Town |
| Lackawanna | 14 | 19,553 | 19,931 | -0.39% | 2,986 | 7 | City |
| Evans | 15 | 15,131 | 15,295 | -0.01% | 364 | 42 | Town |
| Eggertsville | 16 | 15,090 | 14,806 | 0.03% | 5,274 | 3 | CDP |
| Tonawanda | 17 | 14,948 | 15,125 | -0.24% | 3,931 | 4 | City |
| Kenmore | 18 | 14,876 | 15,174 | -0.4% | 10,359 | 1 | Village |
| Depew | 19 | 14,850 | 15,149 | -0.4% | 2,926 | 5 | Village |
| Elma | 20 | 11,735 | 11,711 | 0.3% | 340 | 35 | Town |
| Alden | 21 | 10,483 | 9,673 | 2.79% | 305 | 34 | Town |
| Lancaster | 22 | 10,075 | 10,170 | -0.19% | 3,707 | 3 | Village |
| Hamburg | 23 | 9,856 | 9,750 | 0.21% | 3,976 | 2 | Village |
| Newstead | 24 | 8,719 | 8,690 | 0.31% | 172 | 51 | Town |
| Concord | 25 | 8,302 | 8,304 | 0.47% | 119 | 70 | Town |
| Boston | 26 | 7,956 | 7,948 | 0.1% | 222 | 36 | Town |
| Eden | 27 | 7,571 | 7,567 | 0.32% | 190 | 40 | Town |
| Harris Hill | 28 | 6,257 | 5,982 | 0.81% | 1,549 | 4 | CDP |
| University Buffalo | 29 | 6,220 | 6,079 | 0.16% | 4,607 | 1 | CDP |
| East Aurora | 30 | 5,989 | 5,972 | 0.05% | 2,385 | 3 | Village |
| Williamsville | 31 | 5,508 | 5,411 | 0.35% | 4,378 | 1 | Village |
| Grandyle Village | 32 | 5,159 | 5,099 | 0.68% | 2,743 | 2 | CDP |
| Marilla | 33 | 5,144 | 5,176 | -0.02% | 187 | 28 | Town |
| Highland-on-the-Lake | 34 | 4,761 | 3,953 | 7.13% | 2,335 | 2 | CDP |
| Collins | 35 | 4,509 | 5,672 | 4.18% | 94 | 48 | Town |
| Springville | 36 | 4,189 | 4,212 | -0.12% | 1,138 | 4 | Village |
| Sloan | 37 | 3,720 | 3,770 | -0.27% | 4,769 | 1 | Village |
| Elma Center | 38 | 3,650 | 3,806 | 2.01% | 581 | 6 | CDP |
| North Collins | 39 | 3,519 | 3,500 | 0.17% | 82 | 43 | Town |
| Clarence Center | 40 | 3,516 | 3,211 | 2.45% | 1,557 | 2 | CDP |
| Holland | 41 | 3,260 | 3,275 | 0.09% | 91 | 36 | Town |
| Colden | 42 | 3,198 | 3,123 | 0.66% | 90 | 36 | Town |
| Wanakah | 43 | 3,148 | 3,296 | -0.1% | 2,615 | 1 | CDP |
| Lake Erie Beach | 44 | 3,135 | 3,374 | -1.54% | 820 | 4 | CDP |
| Eden | 45 | 3,067 | 3,157 | -0.97% | 548 | 6 | CDP |
| Wales | 46 | 3,035 | 3,009 | 0.5% | 85 | 36 | Town |
| Orchard Park | 47 | 3,034 | 3,091 | -0.39% | 2,244 | 1 | Village |
| Akron | 48 | 2,880 | 2,909 | -0.21% | 1,431 | 2 | Village |
| Alden | 49 | 2,804 | 2,602 | 1.45% | 1,032 | 3 | Village |
| Sardinia | 50 | 2,709 | 2,712 | 0.22% | 54 | 50 | Town |
| Blasdell | 51 | 2,563 | 2,533 | 0.23% | 2,280 | 1 | Village |
| North Boston | 52 | 2,562 | 2,437 | 0.08% | 621 | 4 | CDP |
| Clarence | 53 | 2,492 | 2,238 | -0.4% | 876 | 3 | CDP |
| Billington Heights | 54 | 2,035 | 1,503 | 1.14% | 644 | 3 | CDP |
| Angola | 55 | 2,020 | 2,048 | -0.3% | 1,426 | 1 | Village |
| Brant | 56 | 1,935 | 1,913 | 0.21% | 80 | 24 | Town |
| Town Line | 57 | 1,847 | 1,752 | -1.86% | 400 | 5 | CDP |
| Angola Lake | 58 | 1,631 | 1,682 | -0.18% | 641 | 3 | CDP |
| North Collins | 59 | 1,271 | 1,261 | 0.16% | 1,587 | 1 | Village |
| Holland | 60 | 1,221 | 1,249 | 0.08% | 305 | 4 | CDP |
| Farnham | 61 | 362 | 365 | -0.28% | 300 | 1 | Village |
Buffalo is the largest city in Erie County, with a population of 276,381.
Farnham is the smallest city in Erie County, with a population of 362.
There are 61 cities in Erie County, New York.