35 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Owego | 1 | 18,801 | 18,781 | 0.18% | 180 | 104 | Town |
| Owego | 2 | 18,801 | 18,750 | 0.18% | 180 | 104 | Town |
| Barton | 3 | 8,355 | 8,614 | -0.23% | 141 | 59 | Town |
| Barton | 4 | 8,355 | 8,573 | -0.23% | 141 | 59 | Town |
| Candor | 5 | 4,973 | 5,148 | -0.24% | 52 | 95 | Town |
| Candor | 6 | 4,973 | 5,138 | -0.24% | 52 | 95 | Town |
| Owego | 7 | 4,297 | 3,656 | 3.12% | 1,686 | 3 | Village |
| Owego | 8 | 4,297 | 3,645 | 3.12% | 1,686 | 3 | Village |
| Tioga | 9 | 4,277 | 4,435 | -0.33% | 73 | 58 | Town |
| Tioga | 10 | 4,277 | 4,430 | -0.33% | 73 | 58 | Town |
| Waverly | 11 | 4,204 | 4,403 | -0.78% | 1,841 | 2 | Village |
| Waverly | 12 | 4,204 | 4,366 | -0.78% | 1,841 | 2 | Village |
| Newark Valley | 13 | 3,504 | 3,642 | -0.2% | 70 | 50 | Town |
| Newark Valley | 14 | 3,504 | 3,635 | -0.2% | 70 | 50 | Town |
| Tioga Terrace | 15 | 2,935 | 2,367 | 7.16% | 2,929 | 1 | CDP |
| Spencer | 16 | 2,868 | 2,964 | -0.14% | 58 | 49 | Town |
| Spencer | 17 | 2,868 | 2,960 | -0.14% | 58 | 49 | Town |
| Apalachin | 18 | 2,662 | 2,148 | 3.98% | 926 | 3 | CDP |
| Nichols | 19 | 2,293 | 2,349 | 0% | 68 | 34 | Town |
| Nichols | 20 | 2,293 | 2,345 | 0% | 68 | 34 | Town |
| Berkshire | 21 | 1,462 | 1,478 | 0% | 48 | 30 | Town |
| Berkshire | 22 | 1,462 | 1,478 | 0% | 48 | 30 | Town |
| Richford | 23 | 1,022 | 1,047 | 0.29% | 27 | 38 | Town |
| Richford | 24 | 1,022 | 1,045 | 0.29% | 27 | 38 | Town |
| Waverly | 25 | 943 | 965 | -0.53% | 8 | 125 | Town |
| Waverly | 26 | 943 | 966 | -0.53% | 8 | 125 | Town |
| Newark Valley | 27 | 886 | 936 | -1.12% | 897 | 1 | Village |
| Newark Valley | 28 | 886 | 935 | -1.12% | 897 | 1 | Village |
| Candor | 29 | 743 | 786 | -1.2% | 1,704 | 0 | Village |
| Candor | 30 | 743 | 785 | -1.2% | 1,704 | 0 | Village |
| Spencer | 31 | 685 | 725 | -1.15% | 662 | 1 | Village |
| Spencer | 32 | 685 | 724 | -1.15% | 662 | 1 | Village |
| Nichols | 33 | 449 | 473 | -1.1% | 867 | 1 | Village |
| Nichols | 34 | 449 | 472 | -1.1% | 867 | 1 | Village |
| West Danby | 35 | 276 | 216 | 6.98% | 202 | 1 | CDP |
Owego is the largest city in Tioga County, with a population of 18,801.
West Danby is the smallest city in Tioga County, with a population of 276.
There are 35 cities in Tioga County, New York.