8 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Windsor | 1 | 3,229 | 3,424 | -1.19% | 1,147 | 3 | Town |
| Aulander | 2 | 709 | 754 | -1.25% | 480 | 1 | Town |
| Lewiston Woodville | 3 | 406 | 435 | -1.46% | 208 | 2 | Town |
| Kelford | 4 | 202 | 211 | -0.98% | 419 | 0 | Town |
| Colerain | 5 | 200 | 215 | -1.48% | 760 | 0 | Town |
| Powellsville | 6 | 176 | 189 | -1.68% | 493 | 0 | Town |
| Roxobel | 7 | 173 | 182 | -1.14% | 166 | 1 | Town |
| Askewville | 8 | 169 | 182 | -1.74% | 285 | 1 | Town |
Windsor is the largest city in Bertie County, with a population of 3,229.
Askewville is the smallest city in Bertie County, with a population of 169.
There are 8 cities in Bertie County, North Carolina.