25 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Athens | 1 | 31,212 | 30,138 | 0.33% | 853 | 37 | Township |
| Athens | 2 | 25,074 | 23,821 | 1.01% | 2,521 | 10 | City |
| York | 3 | 6,836 | 6,840 | 0.4% | 185 | 37 | Township |
| Nelsonville | 4 | 4,543 | 4,600 | -0.26% | 971 | 5 | City |
| Trimble | 5 | 4,055 | 4,065 | 0.4% | 109 | 37 | Township |
| Lee | 6 | 2,857 | 2,844 | 0.49% | 115 | 25 | Township |
| Alexander | 7 | 2,792 | 2,794 | 0.43% | 75 | 37 | Township |
| The Plains | 8 | 2,635 | 2,813 | -1.13% | 1,156 | 2 | CDP |
| Glouster | 9 | 1,642 | 1,665 | -0.3% | 1,254 | 1 | Village |
| Canaan | 10 | 1,637 | 1,630 | 0.49% | 43 | 38 | Township |
| Carthage | 11 | 1,490 | 1,490 | 0.54% | 39 | 39 | Township |
| Ames | 12 | 1,230 | 1,232 | 0.33% | 33 | 38 | Township |
| Albany | 13 | 1,014 | 1,003 | 0.2% | 761 | 1 | Village |
| Chauncey | 14 | 949 | 954 | -0.11% | 1,725 | 1 | Village |
| Bern | 15 | 567 | 568 | 0.53% | 18 | 31 | Township |
| Buchtel | 16 | 518 | 522 | -0.19% | 1,102 | 0 | Village |
| Coolville | 17 | 445 | 452 | -0.45% | 454 | 1 | Village |
| Jacksonville | 18 | 396 | 401 | -0.25% | 1,650 | 0 | Village |
| Athens | 19 | 352 | 371 | -1.4% | 14 | 25 | Township |
| Stewart | 20 | 338 | 326 | 1.81% | 1,142 | 0 | CDP |
| Trimble | 21 | 329 | 328 | 0% | 484 | 1 | Village |
| Millfield | 22 | 237 | 293 | -2.87% | 418 | 1 | CDP |
| Amesville | 23 | 167 | 171 | -0.6% | 736 | 0 | Village |
| New Marshfield | 24 | 148 | 236 | -7.5% | 378 | 0 | CDP |
| Hockingport | 25 | 15 | 144 | -46.43% | 35 | 0 | CDP |
Athens is the largest city in Athens County, with a population of 31,212.
Hockingport is the smallest city in Athens County, with a population of 15.
There are 25 cities in Athens County, Ohio.