33 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Steubenville | 1 | 17,999 | 18,149 | -0.17% | 1,708 | 11 | City |
| Island Creek | 2 | 9,970 | 10,244 | -0.34% | 239 | 42 | Township |
| Cross Creek | 3 | 7,609 | 7,837 | -0.35% | 243 | 31 | Township |
| Toronto | 4 | 5,158 | 5,300 | -0.56% | 2,473 | 2 | City |
| Knox | 5 | 4,212 | 4,310 | -0.26% | 145 | 29 | Township |
| Steubenville | 6 | 3,973 | 4,102 | -0.4% | 530 | 7 | Township |
| Wintersville | 7 | 3,648 | 3,768 | -0.65% | 1,167 | 3 | Village |
| Dillonvale | 8 | 3,277 | 3,411 | -0.4% | 3,674 | 1 | CDP |
| Mingo Junction | 9 | 3,227 | 3,342 | -0.71% | 1,200 | 3 | Village |
| Smithfield | 10 | 2,919 | 3,002 | -0.31% | 78 | 38 | Township |
| Wells | 11 | 2,381 | 2,446 | -0.29% | 89 | 27 | Township |
| Mount Pleasant | 12 | 2,152 | 2,225 | -0.42% | 113 | 19 | Township |
| Tiltonsville | 13 | 1,211 | 1,255 | -0.74% | 2,311 | 1 | Village |
| Saline | 14 | 1,126 | 1,159 | -0.44% | 53 | 21 | Township |
| Richmond | 15 | 997 | 1,011 | -0.3% | 39 | 25 | Township |
| Brilliant | 16 | 964 | 1,302 | -3.5% | 552 | 2 | CDP |
| Richmond | 17 | 945 | 970 | -0.53% | 37 | 25 | Township |
| Yorkville | 18 | 929 | 966 | -0.85% | 1,569 | 1 | Village |
| Adena | 19 | 643 | 667 | -0.77% | 1,197 | 1 | Village |
| Dillonvale | 20 | 592 | 615 | -0.84% | 1,534 | 0 | Village |
| Bergholz | 21 | 519 | 542 | -0.95% | 922 | 1 | Village |
| Amsterdam | 22 | 422 | 437 | -0.71% | 1,319 | 0 | Village |
| Richmond | 23 | 398 | 412 | -0.75% | 720 | 1 | Village |
| Brush Creek | 24 | 382 | 392 | 0% | 16 | 24 | Township |
| Rayland | 25 | 379 | 393 | -0.79% | 801 | 0 | Village |
| Mount Pleasant | 26 | 377 | 392 | -0.79% | 1,478 | 0 | Village |
| Irondale | 27 | 315 | 327 | -0.94% | 217 | 1 | Village |
| Stratton | 28 | 260 | 265 | -0.38% | 484 | 1 | Village |
| Empire | 29 | 226 | 230 | -0.44% | 753 | 0 | Village |
| New Alexandria | 30 | 223 | 232 | -0.89% | 608 | 0 | Village |
| East Springfield | 31 | 139 | 263 | 6.92% | 339 | 0 | CDP |
| Bloomingdale | 32 | 133 | 140 | -1.48% | 1,511 | 0 | Village |
| Pottery Addition | 33 | 52 | 139 | -23.53% | 61 | 1 | CDP |
Steubenville is the largest city in Jefferson County, with a population of 17,999.
Pottery Addition is the smallest city in Jefferson County, with a population of 52.
There are 33 cities in Jefferson County, Ohio.