10 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Florence | 1 | 41,129 | 40,096 | 0.5% | 1,679 | 24 | City |
| Lake City | 2 | 5,887 | 6,030 | -0.49% | 1,080 | 5 | City |
| Timmonsville | 3 | 2,121 | 2,098 | 0.19% | 764 | 3 | Town |
| Johnsonville | 4 | 1,348 | 1,356 | -0.15% | 637 | 2 | City |
| Pamplico | 5 | 1,061 | 1,060 | 0% | 655 | 2 | Town |
| Coward | 6 | 716 | 710 | 0.14% | 203 | 4 | Town |
| Scranton | 7 | 656 | 653 | 0% | 828 | 1 | Town |
| Danwood | 8 | 588 | 361 | 7.3% | 310 | 2 | CDP |
| Olanta | 9 | 549 | 553 | -0.18% | 552 | 1 | Town |
| Stuckey | 10 | 187 | 196 | -1.06% | 200 | 1 | Town |
Florence is the largest city in Florence County, with a population of 41,129.
Stuckey is the smallest city in Florence County, with a population of 187.
There are 10 cities in Florence County, South Carolina.