34 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Brookings | 1 | 24,851 | 23,371 | 1.21% | 1,781 | 14 | City |
| Volga | 2 | 2,448 | 2,108 | 2.86% | 2,140 | 1 | City |
| Medary | 3 | 1,554 | 1,433 | 1.44% | 47 | 33 | Township |
| Aurora | 4 | 1,185 | 1,055 | 2.24% | 1,416 | 1 | Town |
| Elkton | 5 | 783 | 755 | 0.64% | 524 | 1 | City |
| White | 6 | 559 | 531 | 0.9% | 640 | 1 | City |
| Brookings | 7 | 454 | 425 | 1.11% | 17 | 27 | Township |
| Sterling | 8 | 425 | 399 | 1.19% | 12 | 35 | Township |
| Lake Poinsett | 9 | 424 | 480 | -1.17% | 87 | 5 | CDP |
| Volga | 10 | 408 | 379 | 1.24% | 12 | 34 | Township |
| Aurora | 11 | 302 | 280 | 1.34% | 9 | 32 | Township |
| Alton | 12 | 265 | 245 | 1.53% | 8 | 35 | Township |
| Sherman | 13 | 265 | 248 | 0.76% | 8 | 35 | Township |
| Lake Hendricks | 14 | 261 | 243 | 1.16% | 8 | 31 | Township |
| Afton | 15 | 232 | 214 | 1.75% | 7 | 35 | Township |
| Oslo | 16 | 231 | 211 | 1.76% | 6 | 36 | Township |
| Argo | 17 | 229 | 210 | 2.23% | 6 | 36 | Township |
| Bruce | 18 | 217 | 210 | 0.46% | 586 | 0 | City |
| Oakwood | 19 | 209 | 193 | 1.95% | 6 | 32 | Township |
| Trenton | 20 | 185 | 170 | 1.65% | 5 | 35 | Township |
| Laketon | 21 | 180 | 168 | 1.12% | 5 | 34 | Township |
| Lake Sinai | 22 | 178 | 167 | 0.56% | 5 | 33 | Township |
| Richland | 23 | 177 | 165 | 0.57% | 5 | 33 | Township |
| Bangor | 24 | 172 | 161 | 0.58% | 5 | 35 | Township |
| Parnell | 25 | 167 | 156 | 0.6% | 5 | 36 | Township |
| Preston | 26 | 164 | 154 | 0.61% | 5 | 35 | Township |
| Winsor | 27 | 158 | 148 | 0.64% | 4 | 36 | Township |
| Elkton | 28 | 115 | 108 | 0.88% | 6 | 20 | Township |
| Aurora | 29 | 106 | 106 | -1.85% | 3 | 36 | Township |
| Sinai | 30 | 101 | 94 | 1% | 274 | 0 | Town |
| Oak Lake | 31 | 99 | 93 | 1.02% | 3 | 36 | Township |
| White | 32 | 92 | 88 | 0% | 3 | 36 | Township |
| Bushnell | 33 | 74 | 72 | 0% | 104 | 1 | Town |
| Hetland | 34 | 18 | 18 | 0% | 158 | 0 | Town |
Brookings is the largest city in Brookings County, with a population of 24,851.
Hetland is the smallest city in Brookings County, with a population of 18.
There are 34 cities in Brookings County, South Dakota.