16 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Ashland | 1 | 7,948 | 7,914 | 0.1% | 595 | 13 | City |
| Ashland | 2 | 7,948 | 7,906 | 0.1% | 595 | 13 | City |
| Mellen | 3 | 683 | 698 | -0.44% | 363 | 2 | City |
| Mellen | 4 | 683 | 697 | -0.44% | 363 | 2 | City |
| Butternut | 5 | 366 | 365 | 0% | 232 | 2 | Village |
| Butternut | 6 | 366 | 365 | 0% | 232 | 2 | Village |
| New Odanah | 7 | 334 | 384 | -2.91% | 138 | 2 | CDP |
| Birch Hill | 8 | 324 | 206 | 0.62% | 523 | 1 | CDP |
| Marengo | 9 | 155 | 137 | 1.97% | 148 | 1 | CDP |
| Bayfront | 10 | 149 | 99 | 7.19% | 105 | 1 | CDP |
| Jolmaville | 11 | 129 | 95 | 7.5% | 10 | 12 | CDP |
| Glidden | 12 | 127 | 231 | -16.45% | 59 | 2 | CDP |
| Franks Field | 13 | 97 | 151 | -3% | 77 | 1 | CDP |
| Odanah | 14 | 79 | 51 | 6.76% | 30 | 3 | CDP |
| Clam Lake | 15 | 61 | 17 | 7.02% | 151 | 0 | CDP |
| Diaperville | 16 | 58 | 21 | 7.41% | 131 | 0 | CDP |
Ashland is the largest city in Ashland County, with a population of 7,948.
Diaperville is the smallest city in Ashland County, with a population of 58.
There are 16 cities in Ashland County, Wisconsin.