14 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Mondovi | 1 | 2,874 | 2,847 | 0.14% | 738 | 4 | City |
| Mondovi | 2 | 2,874 | 2,851 | 0.14% | 738 | 4 | City |
| Buffalo City | 3 | 1,025 | 1,014 | 0.2% | 541 | 2 | City |
| Buffalo City | 4 | 1,025 | 1,014 | 0.2% | 541 | 2 | City |
| Fountain City | 5 | 780 | 805 | -0.64% | 178 | 4 | City |
| Fountain City | 6 | 780 | 805 | -0.64% | 178 | 4 | City |
| Alma | 7 | 731 | 715 | 0.41% | 138 | 5 | City |
| Alma | 8 | 731 | 716 | 0.41% | 138 | 5 | City |
| Cochrane | 9 | 424 | 424 | 0% | 548 | 1 | Village |
| Cochrane | 10 | 424 | 424 | 0% | 548 | 1 | Village |
| Nelson | 11 | 319 | 319 | 0% | 236 | 1 | Village |
| Nelson | 12 | 319 | 319 | 0% | 236 | 1 | Village |
| Gilmanton | 13 | 178 | 122 | 7.23% | 53 | 3 | CDP |
| Waumandee | 14 | 89 | 73 | 7.23% | 511 | 0 | CDP |
Mondovi is the largest city in Buffalo County, with a population of 2,874.
Waumandee is the smallest city in Buffalo County, with a population of 89.
There are 14 cities in Buffalo County, Wisconsin.