26 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Fond Lac | 1 | 44,417 | 44,694 | -0.12% | 2,309 | 19 | City |
| Fond Lac | 2 | 44,417 | 44,674 | -0.12% | 2,309 | 19 | City |
| Ripon | 3 | 7,681 | 7,853 | -0.45% | 1,544 | 5 | City |
| Ripon | 4 | 7,681 | 7,853 | -0.45% | 1,544 | 5 | City |
| North Fond Lac | 5 | 5,428 | 5,384 | 0.17% | 2,551 | 2 | Village |
| North Fond Lac | 6 | 5,428 | 5,383 | 0.17% | 2,551 | 2 | Village |
| Campbellsport | 7 | 2,020 | 1,904 | 1.15% | 1,479 | 1 | Village |
| Campbellsport | 8 | 2,020 | 1,903 | 1.15% | 1,479 | 1 | Village |
| Oakfield | 9 | 1,066 | 1,053 | 0.19% | 1,084 | 1 | Village |
| Oakfield | 10 | 1,066 | 1,055 | 0.19% | 1,084 | 1 | Village |
| Rosendale | 11 | 1,035 | 1,043 | -0.19% | 831 | 1 | Village |
| Rosendale | 12 | 1,035 | 1,042 | -0.19% | 831 | 1 | Village |
| Eden | 13 | 907 | 887 | 0.33% | 1,517 | 1 | Village |
| Eden | 14 | 907 | 889 | 0.33% | 1,517 | 1 | Village |
| Butte Morts | 15 | 893 | 981 | -0.56% | 724 | 1 | CDP |
| Brandon | 16 | 876 | 882 | -0.11% | 1,159 | 1 | Village |
| Brandon | 17 | 876 | 881 | -0.11% | 1,159 | 1 | Village |
| Van Dyne | 18 | 830 | 236 | 4.53% | 1,053 | 1 | CDP |
| Taycheedah | 19 | 579 | 652 | -1.36% | 739 | 1 | CDP |
| St. Cloud | 20 | 494 | 489 | 0.2% | 537 | 1 | Village |
| St. Cloud | 21 | 494 | 489 | 0.2% | 537 | 1 | Village |
| Mount Calvary | 22 | 488 | 487 | 0% | 462 | 1 | Village |
| Mount Calvary | 23 | 488 | 487 | 0% | 462 | 1 | Village |
| Fairwater | 24 | 346 | 351 | -0.29% | 471 | 1 | Village |
| Fairwater | 25 | 346 | 351 | -0.29% | 471 | 1 | Village |
| Alto | 26 | 131 | 171 | 7.38% | 166 | 1 | CDP |
Fond Lac is the largest city in Fond du Lac County, with a population of 44,417.
Alto is the smallest city in Fond du Lac County, with a population of 131.
There are 26 cities in Fond du Lac County, Wisconsin.