9 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Black River Falls | 1 | 3,575 | 3,514 | 0.34% | 854 | 4 | City |
| Merrillan | 2 | 545 | 565 | -0.73% | 430 | 1 | Village |
| Melrose | 3 | 520 | 543 | -0.95% | 649 | 1 | Village |
| Alma Center | 4 | 468 | 486 | -0.85% | 467 | 1 | Village |
| Taylor | 5 | 464 | 482 | -0.85% | 636 | 1 | Village |
| Mission | 6 | 461 | 307 | 7.21% | 822 | 1 | CDP |
| Hixton | 7 | 438 | 455 | -0.9% | 384 | 1 | Village |
| Sand Pillow | 8 | 306 | 278 | 6.99% | 1,313 | 0 | CDP |
| Millston | 9 | 142 | 108 | 0.71% | 136 | 1 | CDP |
Black River Falls is the largest city in Jackson County, with a population of 3,575.
Millston is the smallest city in Jackson County, with a population of 142.
There are 9 cities in Jackson County, Wisconsin.