22 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Mauston | 1 | 4,095 | 4,347 | -1.25% | 916 | 4 | City |
| Mauston | 2 | 4,095 | 4,352 | -1.25% | 916 | 4 | City |
| New Lisbon | 3 | 2,588 | 2,574 | 0.08% | 947 | 3 | City |
| New Lisbon | 4 | 2,588 | 2,577 | 0.08% | 947 | 3 | City |
| Elroy | 5 | 1,244 | 1,355 | -1.82% | 620 | 2 | City |
| Elroy | 6 | 1,244 | 1,356 | -1.82% | 620 | 2 | City |
| Necedah | 7 | 908 | 929 | -0.66% | 329 | 3 | Village |
| Necedah | 8 | 908 | 936 | -0.66% | 329 | 3 | Village |
| Wonewoc | 9 | 718 | 760 | -1.24% | 643 | 1 | Village |
| Wonewoc | 10 | 718 | 761 | -1.24% | 643 | 1 | Village |
| Camp Douglas | 11 | 623 | 648 | -0.8% | 620 | 1 | Village |
| Camp Douglas | 12 | 623 | 648 | -0.8% | 620 | 1 | Village |
| Lyndon Station | 13 | 472 | 496 | -1.05% | 223 | 2 | Village |
| Lyndon Station | 14 | 472 | 496 | -1.05% | 223 | 2 | Village |
| Ironton | 15 | 258 | 274 | -1.15% | 775 | 0 | Village |
| Ironton | 16 | 258 | 273 | -1.15% | 775 | 0 | Village |
| Union Center | 17 | 217 | 227 | -0.91% | 263 | 1 | Village |
| Union Center | 18 | 217 | 227 | -0.91% | 263 | 1 | Village |
| Hustler | 19 | 161 | 169 | -1.23% | 231 | 1 | Village |
| Hustler | 20 | 161 | 169 | -1.23% | 231 | 1 | Village |
| Yuba | 21 | 50 | 52 | -1.96% | 164 | 0 | Village |
| Yuba | 22 | 50 | 52 | -1.96% | 164 | 0 | Village |
Mauston is the largest city in Juneau County, with a population of 4,095.
Yuba is the smallest city in Juneau County, with a population of 50.
There are 22 cities in Juneau County, Wisconsin.