14 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Manitowoc | 1 | 34,604 | 34,663 | -0.03% | 1,928 | 18 | City |
| Two Rivers | 2 | 11,198 | 11,276 | -0.14% | 1,849 | 6 | City |
| Kiel | 3 | 3,984 | 3,938 | 0.23% | 1,576 | 3 | City |
| Cleveland | 4 | 1,580 | 1,584 | -0.06% | 800 | 2 | Village |
| Mishicot | 5 | 1,421 | 1,430 | -0.14% | 557 | 3 | Village |
| Reedsville | 6 | 1,192 | 1,196 | -0.08% | 983 | 1 | Village |
| Valders | 7 | 956 | 955 | 0% | 664 | 1 | Village |
| Whitelaw | 8 | 747 | 741 | 0.13% | 1,423 | 1 | Village |
| St. Nazianz | 9 | 712 | 714 | -0.14% | 897 | 1 | Village |
| Francis Creek | 10 | 674 | 657 | 0.45% | 593 | 1 | Village |
| Maribel | 11 | 340 | 335 | 0.29% | 297 | 1 | Village |
| Kellnersville | 12 | 311 | 314 | -0.32% | 577 | 1 | Village |
| Collins | 13 | 230 | 209 | 1.77% | 135 | 2 | CDP |
| Clarks Mills | 14 | 163 | 139 | 7.24% | 657 | 0 | CDP |
Manitowoc is the largest city in Manitowoc County, with a population of 34,604.
Clarks Mills is the smallest city in Manitowoc County, with a population of 163.
There are 14 cities in Manitowoc County, Wisconsin.