4 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Macclenny | 1 | 8,387 | 7,303 | 2.56% | 1,724 | 5 | City |
| Macclenny | 2 | 8,387 | 7,342 | 2.56% | 1,724 | 5 | City |
| Glen St. Mary | 3 | 512 | 468 | 1.59% | 1,118 | 0 | Town |
| Glen St. Mary | 4 | 512 | 471 | 1.59% | 1,118 | 0 | Town |
Macclenny is the largest city in Baker County, with a population of 8,387.
Glen St. Mary is the smallest city in Baker County, with a population of 512.
There are 4 cities in Baker County, Florida.