38 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Lakeland | 1 | 127,767 | 113,881 | 2.22% | 1,920 | 67 | City |
| Poinciana | 2 | 73,341 | 68,280 | 1.87% | 1,021 | 72 | CDP |
| Winter Haven | 3 | 62,318 | 50,603 | 3.91% | 1,892 | 33 | City |
| Haines City | 4 | 45,604 | 27,949 | 8.39% | 1,933 | 24 | City |
| Bartow | 5 | 22,711 | 19,574 | 2.84% | 489 | 46 | City |
| Auburndale | 6 | 21,888 | 15,742 | 5.95% | 1,614 | 14 | City |
| Davenport | 7 | 18,522 | 9,730 | 10.49% | 4,211 | 4 | City |
| Lake Wales | 8 | 18,175 | 16,290 | 2.12% | 958 | 19 | City |
| Highland City | 9 | 12,861 | 11,392 | 1.06% | 1,631 | 8 | CDP |
| Medulla | 10 | 11,971 | 11,336 | 1.74% | 2,138 | 6 | CDP |
| Lakeland Highlands | 11 | 11,331 | 11,575 | 0.16% | 2,330 | 5 | CDP |
| Cypress Gardens | 12 | 9,886 | 10,040 | 0.66% | 2,866 | 3 | CDP |
| Lake Alfred | 13 | 9,155 | 6,539 | 6.06% | 931 | 10 | City |
| Inwood | 14 | 7,217 | 7,196 | 0.77% | 3,823 | 2 | CDP |
| Loughman | 15 | 7,200 | 6,762 | 4.36% | 2,073 | 3 | CDP |
| Willow Oak | 16 | 6,923 | 7,168 | 0.19% | 2,220 | 3 | CDP |
| Dundee | 17 | 6,525 | 5,273 | 3.98% | 590 | 11 | Town |
| Jan Phyl Village | 18 | 6,403 | 6,195 | 0.87% | 1,400 | 5 | CDP |
| Eagle Lake | 19 | 6,206 | 3,055 | 11.3% | 2,390 | 3 | City |
| Crystal Lake | 20 | 6,182 | 6,211 | 0.73% | 2,421 | 3 | CDP |
| Kathleen | 21 | 5,721 | 6,402 | -0.71% | 795 | 7 | CDP |
| Fort Meade | 22 | 5,401 | 5,101 | 1.12% | 660 | 8 | City |
| Fussels Corner | 23 | 4,915 | 4,780 | -0.87% | 914 | 5 | CDP |
| Combee Settlement | 24 | 4,706 | 6,173 | -1.22% | 2,382 | 2 | CDP |
| Mulberry | 25 | 4,619 | 3,964 | 2.92% | 679 | 7 | City |
| Wahneta | 26 | 4,235 | 3,988 | -1.33% | 1,862 | 2 | CDP |
| Frostproof | 27 | 3,530 | 3,214 | 1.82% | 309 | 11 | City |
| Grenelefe | 28 | 3,306 | 3,073 | 3.25% | 778 | 4 | CDP |
| Alturas | 29 | 3,279 | 3,778 | -1.8% | 61 | 54 | CDP |
| Polk City | 30 | 3,152 | 2,772 | 2.47% | 700 | 5 | Town |
| Lake Hamilton | 31 | 2,269 | 1,552 | 6.73% | 727 | 3 | Town |
| Crooked Lake Park | 32 | 2,158 | 1,591 | 1.36% | 3,141 | 1 | CDP |
| Babson Park | 33 | 1,000 | 1,451 | -2.34% | 656 | 2 | CDP |
| Indian Lake Estates | 34 | 840 | 686 | 7.14% | 77 | 11 | CDP |
| Bradley Junction | 35 | 757 | 702 | 0.66% | 359 | 2 | CDP |
| Hillcrest Heights | 36 | 290 | 250 | 2.84% | 1,813 | 0 | Town |
| Homeland | 37 | 251 | 231 | -3.09% | 591 | 0 | CDP |
| Waverly | 38 | 144 | 734 | -22.58% | 100 | 1 | CDP |
Lakeland is the largest city in Polk County, with a population of 127,767.
Waverly is the smallest city in Polk County, with a population of 144.
There are 38 cities in Polk County, Florida.