33 cities
| City | County | 2025 Pop. ↓ |
|---|---|---|
| Eldridge | Scott County | 7,026 |
| Estherville | Emmet County | 5,777 |
| Evansdale | Black Hawk County | 4,504 |
| Eagle Grove | Wright County | 3,618 |
| Emmetsburg | Palo Alto County | 3,615 |
| Eldora | Hardin County | 2,581 |
| Ely | Linn County | 2,377 |
| Epworth | Dubuque County | 2,044 |
| Earlham | Madison County | 1,410 |
| Elkader | Clayton County | 1,186 |
| Elkhart | Polk County | 1,126 |
| Elk Run Heights | Black Hawk County | 1,060 |
| Eddyville | Wapello County | 964 |
| Edgewood | Clayton County | 908 |
| Eldon | Wapello County | 788 |
| Exira | Audubon County | 760 |
| Essex | Page County | 720 |
| Earlville | Delaware County | 712 |
| Elgin | Fayette County | 673 |
| Elk Horn | Shelby County | 638 |
| Everly | Clay County | 569 |
| Early | Sac County | 566 |
| Ellsworth | Hamilton County | 492 |
| Elma | Howard County | 489 |
| Emerson | Mills County | 401 |
| Earling | Shelby County | 394 |
| Elliott | Montgomery County | 325 |
| Elberon | Tama County | 177 |
| Exline | Appanoose County | 156 |
| East Peru | Clarke County | 120 |
| East Amana | Iowa County | 44 |
| Elkport | Clayton County | 29 |
| Ellston | Ringgold County | 23 |