9 cities
| City | County | 2025 Pop. ↓ |
|---|---|---|
| Johnson City | Washington County | 74,263 |
| Jackson | Madison County | 69,578 |
| Jefferson City | Jefferson County | 9,165 |
| Jonesborough | Washington County | 6,953 |
| Jasper | Marion County | 3,664 |
| Jacksboro | Campbell County | 2,235 |
| Jamestown | Fentress County | 2,122 |
| Jellico | Campbell County | 2,114 |
| John Sevier | Knox County | 1,041 |