18 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Galena | 1 | 458 | 470 | -0.65% | 26 | 18 | City |
| Fort Yukon | 2 | 406 | 430 | -1.22% | 61 | 7 | City |
| Nenana | 3 | 335 | 355 | -1.18% | 54 | 6 | City |
| Huslia | 4 | 287 | 304 | -1.37% | 18 | 16 | City |
| McGrath | 5 | 281 | 296 | -1.06% | 6 | 43 | City |
| Tanana | 6 | 230 | 242 | -1.29% | 22 | 11 | City |
| Nulato | 7 | 222 | 234 | -1.33% | 5 | 41 | City |
| Grayling | 8 | 197 | 207 | -1.01% | 18 | 11 | City |
| Allakaket | 9 | 169 | 178 | -1.17% | 11 | 16 | City |
| Holy Cross | 10 | 169 | 178 | -1.17% | 6 | 29 | City |
| Kaltag | 11 | 152 | 161 | -1.3% | 7 | 23 | City |
| Ruby | 12 | 129 | 137 | -1.53% | 18 | 7 | City |
| Shageluk | 13 | 93 | 98 | -1.06% | 9 | 10 | City |
| Koyukuk | 14 | 91 | 96 | -1.09% | 16 | 6 | City |
| Nikolai | 15 | 82 | 81 | 0% | 19 | 4 | City |
| Hughes | 16 | 81 | 85 | -1.22% | 27 | 3 | City |
| Anvik | 17 | 70 | 73 | -1.41% | 7 | 9 | City |
| Bettles | 18 | 23 | 25 | -4.17% | 15 | 2 | City |
Galena is the largest city in Yukon-Koyukuk Census Area, with a population of 458.
Bettles is the smallest city in Yukon-Koyukuk Census Area, with a population of 23.
There are 18 cities in Yukon-Koyukuk Census Area, Alaska.