| City | 2025 Pop. ↓ | Growth |
|---|---|---|
| Prague | 1,331,940 | |
| Brno | 380,587 | |
| Pilsen | 164,180 | |
| Havirov | 82,768 | |
| Olomouc | 73,714 | |
| Liberec | 69,663 | |
| Usti nad Labem | 64,179 | |
| Frydek-Mistek | 59,416 | |
| Zlin | 59,364 | |
| Hradec Kralove | 57,118 | |
| Ceske Budejovice | 55,709 | |
| Kladno | 50,470 | |
| Pardubice | 50,211 | |
| Most | 46,328 | |
| Decin | 42,563 | |
| Teplice | 41,891 | |
| Karlovy Vary | 41,136 | |
| Opava | 40,663 | |
| Prostejov | 39,146 | |
| Ceska Lipa | 38,841 | |
| Trebic | 38,785 | |
| Karvina | 38,465 | |
| Tabor | 36,264 | |
| Znojmo | 35,280 | |
| Pribram | 35,251 | |
| Orlova | 34,282 | |
| Cheb | 33,242 | |
| Jablonec nad Nisou | 32,749 | |
| Modrany | 31,901 | |
| Liben | 31,756 | |
| Trutnov | 31,398 | |
| Jihlava | 31,268 | |
| Pisek | 29,774 | |
| Kolin | 29,690 | |
| Kromeriz | 29,126 | |
| Prerov | 29,054 | |
| Chomutov | 28,848 | |
| Sumperk | 28,768 | |
| Vsetin | 28,575 | |
| Valasske Mezirici | 27,481 | |
| Litvinov | 27,022 | |
| Novy Jicin | 26,547 | |
| Uherske Hradiste | 26,421 | |
| Hodonin | 26,345 | |
| Breclav | 25,789 | |
| Cesky Tesin | 25,750 | |
| Krnov | 25,547 | |
| Sokolov | 24,901 | |
| Litomerice | 24,489 | |
| Havlickuv Brod | 24,356 | |
| Zgar nad Sazavou | 24,030 | |
| Zgar nad Sazavou Druhy | 23,996 | |
| Chrudim | 23,630 | |
| Strakonice | 23,545 | |
| Koprivnice | 23,424 | |
| Mlada Boleslav | 23,204 | |
| Klatovy | 23,102 | |
| Bohumin | 23,075 | |
| Stary Bohumin | 23,034 | |
| Jindrichuv Hradec | 22,812 | |
| Vyskov | 22,265 | |
| Trinec | 21,610 | |
| Cerny Most | 21,500 | |
| Kutna Hora | 21,280 | |
| Nachod | 21,263 | |
| Jirkov | 21,056 | |
| Blansko | 20,384 | |
| Zatec | 19,607 | |
| Hranice | 19,582 | |
| Melnik | 19,231 | |
| Louny | 19,147 | |
| Otrokovice | 18,857 | |
| Kadan | 18,759 | |
| Branik | 18,740 | |
| Bruntal | 17,686 | |
| Beroun | 17,550 | |
| Uhersky Brod | 17,508 | |
| Svitavy | 17,427 | |
| Kralupy nad Vltavou | 17,373 | |
| Roznov pod Radhostem | 17,238 | |
| Ostrov | 17,206 | |
| Ceska Trebova | 16,655 | |
| Pelhrimov | 16,541 | |
| Rakovnik | 16,473 | |
| Neratovice | 16,427 | |
| Jicin | 16,328 | |
| Benesov | 16,257 | |
| Dvur Kralove nad Labem | 16,150 | |
| Varnsdorf | 15,895 | |
| Letnany | 15,862 | |
| Bilina | 15,738 | |
| Bilina Kyselka | 15,697 | |
| Prosek | 15,581 | |
| Brandys nad Labem-Stara Boleslav | 15,398 | |
| Usti nad Orlici | 15,151 | |
| Slany | 15,070 | |
| Klasterec nad Ohri | 15,040 | |
| Turnov | 14,507 | |
| Chodov | 14,454 | |
| Nymburk | 14,373 | |
| Zabreh | 14,360 | |
| Horni Pocernice | 14,296 | |
| Marianske Lazne | 14,277 | |
| Hlucin | 14,228 | |
| Cesky Krumlov | 14,146 | |
| Strizkov | 14,069 | |
| Sternberk | 13,967 | |
| Rokycany | 13,826 | |
| Krupka | 13,687 | |
| Podebrady | 13,128 | |
| Roudnice nad Labem | 13,084 | |
| Vrchlabi | 12,898 | |
| As | 12,866 | |
| Jaromer | 12,831 | |
| Tachov | 12,640 | |
| Jesenik | 12,457 | |
| Unicov | 12,385 | |
| Holesov | 12,384 | |
| Vysoke Myto | 12,293 | |
| Vlasim | 12,225 | |
| Kyjov | 12,191 | |
| Novy Bor | 12,171 | |
| Veseli nad Moravou | 12,081 | |
| Karlin | 11,971 | |
| Prachatice | 11,803 | |
| Velke Mezirici | 11,753 | |
| Rychnov nad Kneznou | 11,695 | |
| Vysocany | 11,646 | |
| Susice | 11,483 | |
| Moravska Trebova | 11,414 | |
| Frenstat pod Radhostem | 11,334 | |
| Ricany | 11,329 | |
| Boskovice | 11,121 | |
| Rumburk | 11,101 | |
| Domazlice | 10,944 | |
| Humpolec | 10,914 | |
| Nove Mesto na Morave | 10,537 | |
| Hlinsko | 10,411 | |
| Studenka | 10,341 | |
| Litomysl | 10,146 | |
| Stare Mesto | 10,127 | |
| Nove Mesto nad Metuji | 10,126 | |
| Celakovice | 10,125 | |
| Litovel | 10,062 | |
| Caslav | 10,025 |
